Fig. 1

Experimental workflow. Primary fibroblasts of all donors were processed as matched triplets consisting of a donor with a first primary neoplasm only, a donor with a second primary neoplasm and a cancer-free control. The triplets were exposed to a low dose of ionizing radiation (LDIR, 0.05 Gray), a high dose of ionizing radiation (HDIR, 2 Gray), or were sham-irradiated (0 Gray). mRNA was extracted 4 h after exposure, Illumina-sequenced, and processed for the investigation of differentially expressed genes for each donor with regard to the regulation/level in sham-irradiated cells (dashed arrows). Differentially expressed genes as a result of groupwise-expression models were further subjected to Ingenuity Pathway Analysis and resulting gene-sets of the intra-group comparisons were analyzed for over-representation using the Gene Ontology via ConsensusPathDB