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Fig. 3 | Molecular Medicine

Fig. 3

From: LncRNA KCNQ1OT1 promotes the metastasis of ovarian cancer by increasing the methylation of EIF2B5 promoter

Fig. 3

LncRNA KCNQ1OT1 could decrease EIF2B5 expression by promoting EIF2B5 methylation. A, B RT-qPCR analysis and Western blotting for the mRNA and protein levels of EIF2B5 in SKOV3 and SW626 cells transfected with Control (blank), sh-NC or sh-KCNQ1OT1. C Nuclear-cytoplasmic fractionation assay for the subcellular location of KCNQ1OT1 in SKOV3 and SW626 cells, GAPDH and U6 snRNA serving as internal control for cytoplasmic and nuclear cytoplasmic and nuclear fractions, respectively. D FISH analysis for the subcellular location of KCNQ1OT1 in SKOV3 and SW626 cells. E Prediction for the CpG island in EIF2B5 promoter region by MethPrimer 2.0. F MS-PCR assay for the methylation status of EIF2B5 promoter in SKOV3 and SW626 cells transfected with Control (blank), sh-NC or sh-KCNQ1OT1; M: Methylated primers, U: unmethylated primers. *P < 0.05

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Molecular Medicine

ISSN: 1528-3658