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Fig. 4 | Molecular Medicine

Fig. 4

From: LncRNA KCNQ1OT1 promotes the metastasis of ovarian cancer by increasing the methylation of EIF2B5 promoter

Fig. 4

LncRNA KCNQ1OT1 increased EIF2B5 promoter methylation by recruiting DNA methyltransferases into its promoter region. A, B RT-qPCR analysis and Western blotting for the mRNA and protein levels of DNMT1, DNMT3A and DNMT3B in SKOV3 and SW626 cells transfected with Control (blank), sh-NC or sh-KCNQ1OT1. C Prediction for the binding potency between KCNQ1OT1 and DNMT1, DNMT3A or DNMT3B. D RIP assay for the combining ability of KCNQ1OT1 to DNMT1, DNMT3A and DNMT3B. E ChIP assay for the enrichment of DNMT1, DNMT3A and DNMT3B in EIF2B5 promoter. *P < 0.05

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Molecular Medicine

ISSN: 1528-3658