Table 1 Upstream and downstream primers for target gene detection using PCR
Gene name | Gene primer | Sequence (5ʹ to 3 ʹ) |
|---|---|---|
CDK2 | Forward primer | TCCGGATCTTTCGGACTCTG |
Reverse primer | ACAAGCTCCGTCCATCTTCA | |
CDK6 | Forward primer | TTGTGACAGACATCGACGAG |
Reverse primer | GACAGGTGAGAATGCAGGTT | |
CDK4 | Forward primer | CCAGGCAGGCTTTTCATTCA |
Reverse primer | AGGTCCTGGAAGTATGGGTG | |
Cyclin D | Forward primer | GGGGACAACTCTTAAGTCTCAC |
Reverse primer | CCAATAAAAGACCAATCTCTC | |
Cyclin E | Forward primer | GAGCTTGAATACCCTAGGACTG |
Reverse primer | CGTCTCTCTGTGGAGCTTATAGAC | |
GAPDH | Forward primer | GCCTCGTCTCATAGACAAGATGGT |
Reverse primer | GAAGGCAGCCCTGGTAACC |