Fig. 6
From: Genetic control of neuronal activity enhances axonal growth only on permissive substrates
Neuronal stimulation induces axonal sprouting before the lesion. A Representative images of traced (Dextran-Alexa594) CST and ChR2-YFP in stimulated and non-stimulated Thy1-ChR2 mice in spinal cord sections 500 μm rostral to the lesion core. Scale bar: 100 μm. B Close-up higher magnification images from indicated regions on A. White arrows indicate double-positive Dextran-Alexa 594/ChR2-YFP sprouted axons. Scale bar: 50 μm. C The number of double-positive Dextran-Alexa 594/ChR2-YFP sprouted axons in the grey matter as a normalized ratio of Dextran-Alexa 594-traced CST axons shows a significantly higher percentage of sprouted axons in stimulated animals at distances up to 400 µm laterally and ventrally from CST. Data are expressed as mean ± s.e.m. at each distance from the CST (n = 4 mice) ***p < 0.001; **p < 0.01 denote significant differences in ANOVA followed by Bonferroni test