Fig. 2

HSA-treated tubular epithelial cells promoted macrophage glycolysis. Macrophages were co-cultured with HK-2 cells. A mRNA expression of GLUT1, HK2 and LDHA; B, C protein levels of HK2 and LDHA (n = 3); D amount of lactate in the macrophage supernatant (n = 3); *p < 0.05 vs. the co-control group; #p < 0.05 vs. the co-control + LPS group. HK-2 cells were transfected with Rab27a siRNA: E mRNA levels of Rab27a in HK-2 cells (n = 3); F EV protein levels of CD63 and Tsg101 from the same number of HK-2 cells; G total EV protein from the same number of tubular epithelial cell-derived EVs (n = 3); *p < 0.05 vs. the Si-NC group. Macrophages were co-cultured with HK-2 cells transfected with Rab27a siRNA or Si-NC: H mRNA levels of GLUT1, HK2 and LDHA (n = 3); I, J protein levels of HK2 and LDHA (n = 3); K amount of lactate in the macrophage supernatant (n = 3); *p < 0.05 vs. the co-HSA-Si-NC + LPS group