Fig. 7

Activation of Notch signaling reversed the negative effects of M1 macrophages on osteocyte mineralization. A(i) Alizarin Red S staining images of M1 macrophage-stimulated osteocytes with or without Notch activation; A(ii) the bar graph showed the average OD value of Alizarin Red S staining; OD values were shown as mean ± SD (*p < 0.05, one-way ANOVA); B SEM images of mineralized collagen fibers synthesized from M1 macrophage-stimulated osteocytes with or without activation of Notch signaling pathway (Top: images captured by SE2 mode; Bottom: DDC-SEM images; scale bars represented 1 μm); C Ca/P ratio of mineralized collagen from the above samples detected with EDS; D TEM images and X-ray diffraction patterns demonstrated the ultrastructure of mineralized collagen synthesized by macrophage-stimulated osteocytes with or without Notch signaling pathway activation