Fig. 2

LINC00240 promotes function of trophoblasts and M2 macrophage polarization in preeclampsia model in vitro. A qPCR measurement on LINC00240 levels in two human trophoblasts (HTR-8/SVneo and JEG-3) cultured in a normal or hypoxic condition. B qPCR measurement on LINC00240 levels in trophoblasts under a normal or hypoxic condition with/without LINC00240 overexpression. C CCK-8 measurement on cell proliferation in indicated groups. D Colony formation assay on cell proliferation in indicated groups. E Wound healing assay on cell migration in indicated groups. F Flow cytometry analysis of M2 polarization markers (Arg-1, CD68) in macrophages co-cultured with trophoblasts after indicated treatments. G qPCR measurement on M2 marker (Arg-1) mRNA levels in macrophages co-cultured with trophoblasts after indicated treatments. H qPCR measurement on M1 marker (iNOS) mRNA levels in macrophages co-cultured with trophoblasts after indicated treatments. All the experiments were repeated at least three times. *P < 0.05, **P < 0.01 and ***P < 0.001