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Fig. 3 | Molecular Medicine

Fig. 3

From: LINC00240/miR-155 axis regulates function of trophoblasts and M2 macrophage polarization via modulating oxidative stress-induced pyroptosis in preeclampsia

Fig. 3

LINC00240 negatively regulates miR-155 expression in trophoblasts. A qPCR measurement on LINC00240 expression levels in HTR-8/SVneo and JEG-3 cells with LINC00240 overexpression (OE-LINC00240) or LINC00240 knockdown (sh-LINC00240). B qPCR measurement on miR-155, miR-26a, miR-26b, miR-124, and miR-625 expression levels in HTR-8/SVneo and JEG-3 cells with LINC00240 overexpression or knockdown. C Bioinformatics analysis of the possible binding sites between LINC00240 and miR-155. D Measurement of luciferase activities of wild type (WT) or mutated (MUT) LINC00240 using dual luciferase assay with enhanced or inhibited miR-155 expression. E Detection of direct binding between LINC00240 and miR-155 using RIP assay. F qPCR measurement on miR-155 expression levels in placenta tissues from preeclampsia patients (n = 24) and normal pregnant women (n = 24). G A correlation analysis between LINC00240 and miR-155 levels in placenta tissues from preeclampsia patients (n = 24). H qPCR measurement on miR-155 expression levels in HTR-8/SVneo and JEG-3 cells cultured in a normal or hypoxic condition. All the experiments were repeated at least three times. *P < 0.05 and **P < 0.01

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Molecular Medicine

ISSN: 1528-3658