Fig. 4

LINC00240 inhibits oxidative stress-induced pyroptosis of trophoblasts in preeclampsia model in vitro by negatively targeting miR-155. A LINC00240 levels were measured using qPCR in HTR-8/SVneo cells after indicated treatments. B MiR-155 levels were measured using qPCR in HTR-8/SVneo cells after indicated treatments. C Nrf2 mRNA levels were measured using qPCR in HTR-8/SVneo cells after indicated treatments. D Nrf2 protein levels were measured by western blotting in HTR-8/SVneo cells after indicated treatments. E ROS and MDA levels were measured using ROS assay Kit and MDA assay Kit, respectively. F Protein levels of HO-1, NQO1 and eNOS were measured by western blotting. G Caspase-1, a pyroptosis marker, was measured using flow cytometry. H Protein levels of pyroptosis markers (caspase-1, NLRP3, ASC and HMGB1) were measured by western blotting. I HMGB1 protein levels were measured using ELISA. All the experiments were repeated at least three times. *P < 0.05, **P < 0.01 and ***P < 0.001