Fig. 5

LINC00240 improves function of trophoblasts and M2 macrophage polarization in preeclampsia model in vitro by negatively targeting miR-155. A M2 macrophage marker (Arg-1, CD68) levels were measured by flow cytometry in macrophages co-cultured with trophoblasts after indicated treatments. B Arg-1 expression levels were measured by qPCR in macrophages co-cultured with trophoblasts after indicated treatments. C iNOS expression levels were measured by qPCR in macrophages co-cultured with trophoblasts after indicated treatments. D Cell proliferation was measured by CCK-8 assay in trophoblasts after indicated treatments. E Cell proliferation was measured by colony formation assay in trophoblasts after indicated treatments. F Cell migration ability was assessed using wound healing assay in trophoblasts after indicated treatments. G Cell invasion ability was measured using Transwell assay in trophoblasts after indicated treatments. All the experiments were repeated at least three times. *P < 0.05, **P < 0.01 and ***P < 0.001