Fig. 6

MiR-155 negatively regulates Nrf2 expression in trophoblasts. A qPCR measurement of Nrf2 mRNA levels in placenta tissues from preeclampsia patients (n = 24) and normal pregnant women (n = 24). B Western blot analysis of Nrf2 protein expression in placenta tissues from preeclampsia patients and normal pregnant women (n = 12, randomly selected from 24 placenta tissues from preeclampsia patients and 24 placenta tissues from normal pregnant women). C qPCR measurement of Nrf2 mRNA levels in HTR-8/SVneo and JEG-3 cells cultured under a normal or hypoxic condition. D A correlation analysis between miR-155 and Nrf2 mRNA levels in placenta tissues from preeclampsia patients (n = 24). E qPCR measurement of miR-155 levels in HTR-8/SVneo and JEG-3 cells transfected with miR-155 mimics or mimics NC. F Western blot analysis of Nrf2 protein expression in HTR-8/SVneo and JEG-3 cells transfected with miR-155 mimics or mimics NC. G qPCR measurement of miR-155 levels in HTR-8/SVneo and JEG-3 cells transfected with miR-155 inhibitor or inhibitor NC. H Western blot analysis of Nrf2 protein expression in HTR-8/SVneo and JEG-3 cells transfected with miR-155 inhibitor or inhibitor NC. I Bioinformatics analysis of the binding sites between miR-155 and Nrf2. J Measurement of luciferase activities of wild type (WT) or mutated (MUT) Nrf2 using dual luciferase assay with enhanced or inhibited miR-155 expression. All the experiments were repeated at least three times. *P < 0.05 and **P < 0.01