Fig. 7

PNU decreased the expression levels of NF-κB and MAPK in RAW264.7 cells. The expression levels of MAPK and NF-κB pathway-related proteins were detected by Western blot. A Western blot assays were performed to detect the protein expression levels of IKBα, p-IKBα, p-P65, p-ERK and GAPDH in RAW264.7 cells. B Relative Western blot protein intensity. C The effect of PNU-282987 on the nuclear translocation of NF-κB p65 was measured by immunofluorescence. Blue fluorescence represents the cell nucleus, and red fluorescence represents p65. The p65 subunit translocated into the nucleus after LPS stimulation; PNU reduced p65 nuclear translocation caused by LPS (scale bar: 50 μm). D Immunofluorescence staining quantitative relative area