Fig. 9From: Protective effect of α7 nicotinic acetylcholine receptor activation on experimental colitis and its mechanismPNU decreased the phagocytosis of macrophages in colon tissues of mice and RAW264.7 cells. The phagocytic function of macrophages was detected by immunofluorescence and flow cytometry. A Immunostaining assay was performed to detect E-coli phagocytosis of macrophages in RAW264.7 cells. Scale bar, 100 μm. B Immunofluorescence staining quantitative relative area. C Phagocytosis was analyzed by flow cytometry. D The rate of phagocytosis was calculated using the median fluorescence intensity (MFI). Data are expressed as the mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001Back to article page