Fig. 3

The renoprotection of spermidine largely relies on macrophage. Clodronate liposome (CL) was administered intravenously 24 h before LPS injection to deplete macrophages. Empty liposome (EL) was used as control. Spermidine was administered intraperitoneally following LPS injection. Control n = 6, EL + LPS + Vehicle n = 6, EL + LPS + SPD n = 7, CL + LPS + Vehicle n = 5, CL + LPS + SPD n = 5. Levels of A serum creatinine (Cr) and B blood urea nitrogen (BUN) were detected. C Representative images of HE staining are exhibited, and tubular injury scores were calculated. Black arrow indicated the tubular vacuolation. Scale bar, 100 μm. Renal mRNA levels of D KIM-1, E NGAL, F IL-1β, G IL-6, H TNF-α, I MCP-1, and J ICAM-1 were analyzed by qPCR. Data are expressed as mean ± SEM. Two-way ANOVA followed by post hoc Sidak test. *P < 0.05, **P < 0.01, ***P < 0.001