Fig. 6
Inhibition of eIF5AH partially abrogated the effects of spermidine on macrophages. Spermidine was administered in the presence or absence of GC7 or CPX, both of which are inhibitors for eIF5A hypusination. A ROS (red) and Hoechst (blue) staining. Scale bar, 100 μm. B JC-1 staining. Scale bar, 100 μm. Cellular mRNA levels of C IL-1β, D IL-6, and E MCP-1. Concentrations of F IL-1β, G IL-6, and H MCP-1 in the culture supernatants. Experiments were performed in triplicate. Data are expressed as mean ± SEM. Two-way ANOVA followed by post hoc Sidak test. *P < 0.05, **P < 0.01, *** P < 0.001