Fig. 3

eIF4A specific high throughput screen identifies small molecules with inhibitory effect. A Scatterplot of primary screen results. A total of 29 compounds were tested and luciferase signal reduced by ≥ 50% compared to control were identified and considered active. Luciferase activity results are expressed relative to values obtained in the presence of vehicle controls. Percentage inhibition was calculated and plotted in a scatter plot, n = 3 biological replicates performed Mean ± SEM. B Structure of RBF98, a candidate inhibitor. C Percentage inhibition observed in the treatment of RBF98 at various concentrations in eIF4A-3X-Luciferase Hek293T/17. D Effect of RBF98 on DLBCL colony formation. Representative image of the colony formation in OCI-Ly3 (malignant) and GMO17220B (non-malignant) cells. The total number of colonies grown in E OCI-Ly3 and F GMO17220B cells upon treatment with 0.5 and 1 µM of RBF98. Statistical analysis was performed using one-way ANOVA followed by Bonferroni’s correction analysis. ^ap < 0.05; ^cp < 0.001, ^dp < 0.0001 vs DMSO control groups, ^αp < 0.05, ^βp < 0.001, ^¥p < 0.0001 vs 1 mM RBF98 treated groups