Fig. 6

RBF197 and RBF208 decreases overall translation and eIF4A1 dependent pathway proteins in double-hit lymphoma. A SUnSET assay was performed by exposing cells to puromycin (1 μg/mL), post compound treatments for 30 min, and subsequently lysed. Representative immunoblots were probed with anti-puromycin, anti-eIF4A, and anti-eIF4E. GAPDH was probed as an internal loading control. B Relative fold change in expression levels with on treatment of RBF197 or RBF208 at 1 and 3 µM concentrations. C Representative immunoblots of cMYC, MCL1, PARP1, BCL2, CDK7, NRF2, Cyclin E and CARD11 on treatment with RBF197 and RBF208 at 1 and 3 µM concentrations respectively. Vinculin was used as a loading control. D Relative fold change in protein levels of the above-mentioned proteins with on treatment of RBF197 and RBF208, respectively. E Heat map of translation efficiency values for RBF197 and RBF208 in RC cell line. F Effect of RBF197 and RBF208 on DLBCL colony formation. Representative image of the colony formation in RC (malignant) and GMO13604 (non-malignant) cells. G The total number of colonies grown in RC, and GMO13604 cells upon treatment with 1 and 3 µM of RBF197 and RBF 208, respectively. All treated groups were normalized with DMSO controls and expressed as mean ± SD (n = 3). Statistical analysis was performed using one-way ANOVA followed by Bonferroni’s correction analysis. ^ap < 0.05; ^cp < 0.001, ^dp < 0.0001 vs DMSO control groups, ^αp < 0.05, ^βp < 0.001, ^¥p < 0.0001 vs 3 μM treatment groups