Fig. 2
S9 inhibits cancer cell growth in vitro and in vivo. A U2OS, 786-O, ACHN, HCT116, A549 and HEPG2 were treated with various concentrations of S9 for 24 h. Cell Viability was measured by CCK-8 assay. The inhibition of cell proliferation was achieved by comparing the values of each group with the control. Error bars represent SDs of at least three independent measurements. B Treatment schedule of the in vivo antitumor experiment. C Relative changes in tumor volume versus time. HCT116 (5 × 106) cells were injected subcutaneously into flank sites of BALB/c nude mice. Seven days later, mice were injected 5 mg/kg, 25 mg/kg S9 or PBS by tail vein injection once every other day for six times. D Relative changes in body weight versus time. Data represent mean ± SD (n = 4). *P < 0.05; **P < 0.01; ***P < 0.001 in comparison with PBS group