Fig. 3

S9 causes G2/M-phase cell cycle arrest and apoptosis in cancer cells. A U2OS cells were treated with described concentration of S9 for 24 h, stained with PI and analyzed by flow cytometry. The dataset is representative example of triplicate experiments. Column graph was mean ± SD of three independent experiments. B U2OS cells were treated with described concentration of S9 for 24 h, stained with EDU and DAPI. The red color indicates EDU-positive nuclei. The statistical analysis of EDU staining was performed by Image-Pro Plus 6.0 software. The dataset is representative example of triplicate experiments. Column graph was mean ± SD of three independent experiments. C U2OS cells were treated with described concentration of S9 for 24 h and apoptotic cells quantitatively detected by flow cytometry. The dataset is representative example of triplicate experiments. Column graph was mean ± SD of three independent experiments. D, E U2OS cells were treated with described concentration of S9 with (E) or without QVD (D) (10 μM) for 24 h, PARP cleavage was detected by western blot analysis. The dataset is representative example of triplicate experiments. Data are presented as mean ± SD (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001 in comparison with control group