Fig. 2
From: Acid ceramidase targeting pyruvate kinase affected trypsinogen activation in acute pancreatitis
Acid ceramidase (ASAH1) positively regulated the activity of trypsin and cathepsin B. Lentivirus particles containing nontargeting shRNA or shRNA targeting ASAH1 (A, B, E, G) and control vector or plasmid encoding ASAH1 (A, C, D, F, H, I) were added to primary pancreatic acinar cells. Additionally, the cells were treated with or without 200 nM of cerulein plus 10 ng/mL of LPS (C + L) for 6 h. Cells were lysed, a proportion of lysate was examined by trypsin and cathepsin B activity assay using a microplate reader (A, C, E, F), and the other proportion was subjected to Western blotting analysis with indicated antibodies (G, H, I). Cells were incubated with trypsin rhodamine-conjugated substrate and were visualized with a fluorescence microscope (B, D)