Fig. 4
From: Acid ceramidase targeting pyruvate kinase affected trypsinogen activation in acute pancreatitis
Acid ceramidase (ASAH1) regulated the activity of trypsin and cathepsin B through sphingosine (SPH). Lentivirus particles containing nontargeting shRNA/Control vector or shRNA targeting ASAH1/ASAH1 expressing plasmid were added to primary pancreatic acinar cells. Additionally, the cells were treated as indicated. Cells were fixed, and immunostaining was performed with an anti-ceramide antibody (A). Trypsin activity was detected by a spectrophotometer (B) or fluorescence microscope (C). Cathepsin B activity was detected by a spectrophotometer (D) or Western blotting (E, F)