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Fig. 5 | Molecular Medicine

Fig. 5

From: The interaction between autophagy and the epithelial-mesenchymal transition mediated by NICD/ULK1 is involved in the formation of diabetic cataracts

Fig. 5

Autophagy promoted Notch1 degradation through the interactions of Notch1, LC3 and SQSTM1/p62. HLE-B3 cells were cultured in DMEM (5.5 mM glucose) for 24 h. A Immunofluorescence staining for LC3 (red) and Notch1 (green) in HLE-B3 cells, bar = 40 μm. B Immunofluorescence staining for SQSTM1/p62 (red) and Notch1 (green) in HLE-B3 cells, bar = 40 μm. C, D Co-IP of Notch1, LC3 and SQSTM1/p62. IP: immunoprecipitation, WB: Western blot, n = 3

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Molecular Medicine

ISSN: 1528-3658