Fig. 5
From: ZNF384–ZEB1 feedback loop regulates breast cancer metastasis
ZNF384 promotes breast cancer progression by transactivation of ZEB1 expression. A Four potential ZNF384-binding sites located in ZEB1 promoter region. B The interaction between ZNF384 and ZEB1 promoter region in 293FT cells verified by ChIP analysis. C The effect of ZNF384 expression on ZEB1 promoter activity determined by dual-luciferase reporter analysis in 293FT cells. A series of fragments containing the predicted ZNF384 binding site is fused upstream of Luc gene. D ZEB1 expression determined by western blot in ZNF384-overexpressed T47D cells or ZNF384-depleted MDA-MB-231 cells, as well as control cells. E–F The ability of cell migration and invasion in ZNF384-depleted MDA-MB-231 with or without ZEB1 overexpression determined by transwell analysis. *P < 0.05