Fig. 4
From: PACS-2 deficiency in tubular cells aggravates lipid-related kidney injury in diabetic kidney disease
Identification of Soat1 as a downstream molecule of Pacs-2. A Volcano plot of DEGs in kidney cortex of control Pacs-2fl/fl mice and control PT-Pacs-2−/− mice. Splashes represent different genes, and the gray splashes mean genes without significant different expression. The red splashes mean significantly up-regulated genes in control PT-Pacs-2−/− mice, and the blue splashes mean significantly down-regulated genes in control PT-Pacs-2−/− mice. B Heat map of top eight DEGs involved in lipid metabolism; n = 3 mice per group. C Real-time qRT-PCR showing the relative mRNA expression of Soat1. n = 6 mice per group. D Representative immunofluorescence images of SOAT1 (green) in kidney tissues from each group. The nuclei were delineated by DAPI (blue). n = 6 mice per group. Bars = 50 μm. E Semiquantification analysis of protein expression. F Representative western blot bands of SOAT1, n-SREBP1, n-SREBP2 expression in the kidney cortex, n = 6 mice per group. G Relative band density. All data are presented as means ± SD; *p < 0.05, **p < 0.01, ***p < 0.001