Fig. 5
From: PACS-2 deficiency in tubular cells aggravates lipid-related kidney injury in diabetic kidney disease
Treatment of HK-2 cells with PACS-2 and SOAT1 siRNA regulates lipid metabolism. A and B Representative western blot and quantification of PACS-2, SOAT1, n-SREBP1, n-SREBP2 in HK-2 cells. C Measurement of the mRNA expression of selected markers. D Representative images of cells stained with Bodipy (green); nuclei stained with Hoechst (blue). E Quantification of the Bodipy staining. F Cholesteryl ester content. G Free cholesterol content. H Total cholesterol content. I Free fatty acid content. All data are presented as means ± SD; *p < 0.05, **p < 0.01, ***p < 0.001. n = 4