Fig. 6

Effect of miR-351 silencing on the atherosclerotic cell model. A Lentivirus was used to infect cells and deliver packaged shRNA plasmids, and the success of infection was determined by fluorescence microscopy. The scale bar represents 100 μm. B qPCR was used to detect the silencing efficiency of shRNA on the miR-351 gene in the three groups. C MiR-351 was treated with ox-LDL + 30 mM glucose for 48 h, and MTT was used to detect the activity of cells in each group. D DAPI (1 μg/ml) was used for nuclear staining of cells in each group, and apoptotic cells are shown in red circles. The scale bar represents 50 μm. E Flow cytometry was used to detect apoptosis. Annexin V-FITC fluorescence intensity is shown by the horizontal coordinates, and PI staining is shown by the vertical coordinates. Dead cells are present in the R2 quadrant, late apoptotic cells are in the R3 quadrant, normal cells are in the R4 quadrant, and early apoptotic cells are in the R5 quadrant. F Flow cytometry was used to detect apoptosis. G Oil red O stains cells with oil, and red stain indicates the oil-positive part. The scale bar represents 50 μm. **P < 0.01, *P < 0.05, vs. the control group. ^#P < 0.05, vs. LDL + HG group. N = 3 per group