Fig. 8

Gene targets of miR-351. A The localization and expression intensity of miR-351 in cells were detected by FISH. B The relative expression level of the ITGB3 gene in mouse aortic tissue was detected. C The relative expression level of the ITGB3 gene in the atherosclerotic cell model was detected. D The expression level of ITGB3 protein was detected by Western blotting. E The gray values of the Western blotting strips were statistically quantified. F MiR-351-5p and ITGB3 gene 3'UTR targeted binding sequence and ITGB3 gene 3'UTR mutation sequence. G Dual-luciferase activity report detection results. *P < 0.05, ITGB3 WT + miR-351 mimic group vs. any group; H Western blot was used to detect the protein silencing efficiency of ITGB3 after transfection of three siRNA groups. I Statistical quantification of H western blotting results. **P < 0.01, *P < 0.05, vs. control group. Animal and cell experiments, N = 3 per group