Fig. 9

Effect of ITGB3 siRNA transfection on the biological function of miR-351-silenced cells. A The expression levels of ITGB3 and apoptosis-related proteins were detected by western blot. B–E The gray values of ITGB3 Bcl-2, Bax, and active Caspase-3 protein western blot bands were statistically quantified. F Oil red O was used to detect oil accumulation in cells. The scale bar represents 50 μm. G Flow cytometry was used to detect the apoptosis levels of miR-351 cell lines after transfection with ITGB3 siRNA. H The results were statistically quantified by flow cytometry. **P < 0.01, *P < 0.05, vs. the control group. ^#P < 0.05 represents the comparison between the two groups of connections. N = 3 per group