Fig. 4

Ferroptosis inducer RSL3 promotes rat VSMC phenotypic switching. A–D Rat VSMCs were treated with or without RSL3 (80 nM) for 24 h, followed by further analysis. A The representative Western blots and densitometric quantification of SM-MHC, calponin 1, OPN, COX-2, and GPX4 (n = 4–5). B qRT-PCR analysis of Myh11, Acta2, Cnn1, Spp1, Ptgs2 and Gpx4 (n = 3–4). C, D The representative images of α-SMA and GPX4 and quantification of α-SMA MFI and GPX4 relative fluorescence intensity (n = 3). Data are presented as mean ± SEM, P values calculated by a two-tailed unpaired Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001