Fig. 5

Ferroptosis inhibitor Fer-1 reverses RSL3-induced rat VSMC phenotypic switching. A, B Rat VSMCs were pretreated with Fer-1 (0.5 μM) for 15 min and/or RSL3 (80 nM) for an additional 24 h and subjected to the following analyses. A The representative Western blots and densitometric quantification of SM-MHC, calponin 1, and OPN (n = 3 and 4, respectively). B qRT-PCR analysis of Myh11, Acta2, Cnn1, Spp1, and Ptgs2 (n = 3). Data are presented as mean ± SEM, P values calculated by a one-way ANOVA followed by Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01, ***P < 0.001