Fig. 5

c-Jun binds to miR-17-92 promoter DNA. A PROMO online software was applied to predict transcription factor binding sites in the promoter region of the miR-19b gene. c-Jun was identified as a putative transcription factor with two potential binding sites in the miR-17-92 promoter at positions 6237–6243 and 7495–7502. B The binding activity of c-Jun to the promoter of miR-19b was determined by ChiP assay using anti-c-Jun antibody. In the cells exposed to H/R, DNA fragment around positions 7495–7501 was detected by PCR but DNA fragment around 6237–6243 was not, suggesting c-Jun mainly binds to the former binding site. No DNA fragment was detected in the chromatin immunoprecipitants isolated by IgG isotype control. C Summary of the DNA band intensities