Fig. 7

Overexpression conserved sequence TFO2 adenovirus of FENDRR in vivo alleviates the development of HPH and inhibits hypoxia-induced pyroptosis. a Adenoviruses with NC or TFO2 sequences were used to handle mice via dropwise intranasal instillation to mice and subjected to hypoxia until 29 days. b Indices of Right ventricular systolic pressure (RVSP) (n = 10). c Right ventricle (RV)/(left ventricle (LV) + Septum) weight ratio was calculated (n = 10). d HE staining was performed to detect wall thickening (n = 10). e Echocardiographic images and indexes (n = 10). f qRT–PCR was used to examine the lung tissues mRNA levels of Caspase-1, NLRP3 and IL-1β (n = 8). g Immunofluorescence of NLRP3 in mouse lung sections. Scale bars = 50 µm. All values are presented as the mean ± SD. Statistical analysis was performed with one-way ANOVA. NOR: normoxic; HYP: hypoxic; NC: negative control; ns: no significant. *P < 0.05, **P < 0.01, ***P < 0.001 compared with NOR + NC. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 compared with HYP + NC