Table 1 All primers and probes sequences
RT-PCR primers | ||
|---|---|---|
Name | Position | Sequences (5′-3′) |
Hum-FENDRR | Forward primer | CTCCCGTGGAAGCCATTTCT |
Reverse primer | CCTCTGGCTGCGTTTTTCAC | |
Hum-DRP1 | Forward primer | GTTTTTCCATGTAGCAGGGTCA |
Reverse primer | ACCTGCTTCCCAGAGGTACT | |
Mus-Caspase-1 | Forward primer | AGGCACGGGACCTATGTGAT |
Reverse primer | AGGGCAAAACTTGAGGGTCC | |
Mus-NLRP3 | Forward primer | TGGGTTCTGGTCAGACACGAG |
Reverse primer | GGCGGGTAATCTTCCAAATGC | |
Mus-IL-1β | Forward primer | GCCACCTTTTGACAGTGATGAG |
Reverse primer | AAGGTCCACGGGAAAGACAC | |
Hum-β-actin | Forward primer | CTCACCATGGATGATGATATCGC |
Reverse primer | CACATAGGAATCCTTCTGACCCA | |
Mus-β-actin | Forward primer | TGCTTCTAGGCGGACTGTTAC |
Reverse primer | AACCAACTGCTGTCGCCTT | |
DRP1 promoter | Forward primer | CCTCTCCTCACCTGCTTTAATTC |
Reverse primer | GTATCCCTCCTCTACTCCAAACC | |
MSP primers | ||
|---|---|---|
Name | Position | Sequences (5′-3′) |
DRP1 CpG methylation | Forward primer | TAATTTTAATATTTTGGGAGGTCGA |
Reverse primer | CCAAACTAAAATACAATAACGCGAT | |
DRP1 CpG unmethylation | Forward primer | TAATTTTAATATTTTGGGAGGTTGA |
Reverse primer | CCAAACTAAAATACAATAACACAAT | |
Other primers | ||
|---|---|---|
Name | Position | Sequences (5′-3′) |
DRP1 siRNA | Sense | GCCUUAACACUAUUGACAUTT |
Antisense | AUGUCAAUAGUGUUAAGGCTT | |
YTHDC1 siRNA1 | Sense | GGAGAAAGAUGGAGAACUUTT |
Antisense | AAGUUCUCCAUCUUUCUCCTT | |
YTHDC1 siRNA2 | Sense | GCUCUGCAUCAGAGUCAUATT |
Antisense | UAUGACUCUGAUGCAGAGCTT | |
YTHDC1 siRNA3 | Sense | GCAAGGAGUGUUAUCUUAATT |
Antisense | UUAAGAUAACACUCCUUGCTT | |
Negative control | Sense | UUCUCCGAACGUGUCACGUTT |
Antisense | ACGUGACACGUUCGGAGAATT | |
CHIRP probes | ||
|---|---|---|
Negative control | Single strand | UUGUACUCACAAAAGUACUG |
FENDRR TFO1 | Single strand | CGCGCACCUGGCUGCCAGCCCGCAGGGGGCUCGCACGCAGACCUG |
FENDRR TFO2 | Single strand | AGAAGAAAAAACACAAAAUACCCAACCACAGAUCCUCAAAAAUAU |