Fig. 1
From: AAV-ie-mediated UCP2 overexpression accelerates inner hair cell loss during aging in vivo
Oxidative stress occurs in IHCs of ARHL. A Representative IF staining of 4-HNE (red) and 8-OHdG (red) in the cochlea from different groups. IHCs and nuclei were stained with anti-Myosin VIIa (green) and DAPI (blue), respectively. Scale bar, 20 μm. B Representative western blots for UCP2 in the different groups. C Western blot analysis of the relative UCP2 expression (n = 3 independent experiments). D The relative fluorescence intensities of 4-HNE and 8-OHdG, respectively (n = 4 ears/group). E The mean ABR thresholds at four frequencies of 4, 8, 16 and 32 kHz (n = 12 ears/group). F The mean ABR wave I amplitudes at all tested frequencies (n = 12 ears/group). IHC, inner hair cell; ARHL, age-related hearing loss; UCP2, uncoupling protein 2. *p < 0.05, **p < 0.01