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Fig. 2 | Molecular Medicine

Fig. 2

From: SHP-1 knockdown suppresses mitochondrial biogenesis and aggravates mitochondria-dependent apoptosis induced by all trans retinal through the STING/AMPK pathways

Fig. 2

SHP-1 knockdown induces mitochondrial dysfunction. A,B Immunofluorescent images of scramble-rLV- and shRNA-SHP-1-rLV-transfected murine RPE flat mounts, stained for SHP-1/ZO-1 (A), and TOMM20 (B). Scale bar = 50 μm. C Abundance of mitochondria determined by MitoTracker fluorescence intensities in scramble-rLV- and shRNA-SHP-1-rLV-transfected ARPE-19 cells. The scramble-rLV- and shRNA-SHP-1-rLV-transfected ARPE-19 cells were treated with atRAL (7.5 μM, 24 h). D Relative ATP levels. E–G Western blotting analysis of the relative expression levels of Bcl-xL (F) and TOMM20 (G). Representative immunofluorescent images of ARPE-19 cells labeled with TOMM20 were shown in (H). Scale bar = 50 μm. The AR values (I) and FF values (J) were calculated and compared among different groups. *P < 0.05 and **P < 0.01

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Molecular Medicine

ISSN: 1528-3658