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Fig. 3 | Molecular Medicine

Fig. 3

From: SHP-1 knockdown suppresses mitochondrial biogenesis and aggravates mitochondria-dependent apoptosis induced by all trans retinal through the STING/AMPK pathways

Fig. 3

SHP-1 interacts with STING and suppresses its activation through posttranslational modifications. A–C ARPE-19 cells were exposed to atRAL (7.5 μM, 0, 3, 6, 12 h). Immunofluorescent images of cells costained for SHP-1 and calnexin (A) or for SHP-1 and STING (B) were shown. Scale bar = 50 μm. C Immunoprecipitation and western blotting of cell lysates. D, E Scramble-rLV- and shRNA-SHP-1-rLV-transfected ARPE-19 cells were treated with atRAL (7.5 μM, 6 h), and subject to immunofluorescent staining for STING (D) or immunoprecipitation analysis (E). Scale bar = 50 μm. F ARPE-19 cells were incubated with atRAL (7.5 μM, 0–12 h), and the ubiquitination of STING was detected by immunoprecipitation. G the K63- ubiquitination of STING in the scramble-rLV- and shRNA-SHP-1-rLV-transfected ARPE-19 cells exposed to atRAL (7.5 μM, 6 h)

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Molecular Medicine

ISSN: 1528-3658