Fig. 4

Effects of SHP-1 knockdown on cell apoptosis are attenuated by the inhibition of STING. Scramble-rLV- and shRNA-SHP-1-rLV-transfected ARPE-19 cells were treated with atRAL (7.5 μM), with or without pretreatment with the STING antagonist, H151 (1 μM), for the indicated time. A Viability of ARPE-19 cells (atRAL, 24 h) was examined using a CCK-8 assay. B Light microscopic images of the morphological features of ARPE-19 cells (atRAL, 24 h). Scale bar = 100 μm. C, D Representative images of ARPE-19 cells (atRAL, 12 h) stained with Annexin V-FITC/ PI (C), and quantitative analysis of the apoptosis rates (D). E, F ARPE-19 cells (atRAL, 12 h) were stained with JC-1 dyes (E), and the fluorescence intensity ratios (red/green) were calculated (F). Scale bar = 100 μm. G, H Western blotting analysis of Bcl-xL expression levels in ARPE-19 cells (atRAL, 24 h). *P < 0.05 and **P < 0.01