Fig. 2

Inhibition of platelet activation suppressed reactive EGCs. a, b Flow cytometric dot blots of 2-color fluorescence staining of mice platelets with anti-CD40L followed by anti-CD41-FITC. The numbers at corners indicated the percentage of CD41 and CD40L double positive population (n = 4). c Serum sCD40L levels (n = 6). d–f Immunofluorescence staining analysis of GFAP (green) and CD40 (red), Scale bar = 100 μm (n = 6). g–i Western blot analysis of GFAP and CD40 expression (n = 6). The data are presented as the mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001