Fig. 4

Effect of CD40L–CD40–TRAF6 signaling on EGCs in vitro. a, b Primary EGCs were stimulated with LPS, CD40L alone or LPS + CD40L for 24 h siRNA transfection and the EGCs-conditioned medium were collected for Caco-2 culture. Western blot analysis of GFAP, CD40 and TRAF6 expression in EGCs (c, d, g, i), ZO-1 and occludin expression in Caco-2 cells (e, f, h, j) (n = 6). k Immunofluorescence staining analysis of ZO-1 (red), Scale bar = 50 μm. l Transepithelial electrical resistance (n = 3). TNF-α (m) and IL-1β (n) levels in EGCs-conditioned medium (n = 6). The data are presented as the mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001