Fig. 1

Circ_AFF4 and KDM1A were significantly increased in BM-MSCs upon osteoinduction

(A) Protein expression of cell surface markers, CD14, CD45, HLA-DR, CD73, CD90 and CD105, in BM-MSCs was assessed by FACS. (B) Representative images of ARS and ALP staining of BM-MSCs cultured in osteogenic medium (OM) or normal medium (NM) for 14-days. Scar bar = 100 μm. Relative gene (C) and protein (D) expression of osteogenic markers, ALP, RUNX2, OPN and Colla 1, in BM-MSCs cultured in OM or NM for 14-days, were detected by RT-qPCR and western blot, respectively. (E) Relative gene expression of circ_AFF4 and KDM1A were assessed by RT-qPCR in BM-MSCs cultured in OM or NM after 0, 3, 7 and 14 days. (F) Relative protein expression of KDM1A was detected by western blot in BM-MSCs cultured in OM or NM after 14 days. *p < 0.05, **p < 0.001 and ***p < 0.001. Each experiment was performed at least three times independently