Fig. 5
From: CircRNA AFF4 induced by KDM1A promotes osteogenic differentiation through FNDC5/Irisin pathway
Knockdown of KDM1A suppresses osteogenic differentiation of BM-MSCs
(A) BM-MSCs were transfected with shKDM1A_1–4# or shNC and the expression level of KDM1A in shKDM1A_1–4# or shNC transfected BM-MSCs was detected using western blot. BM-MSCs that were transfected with shKDM1A or shNC were cultured in OM or NM for 14 days. Afterward, different assays were performed to assess the osteogenic differentiation. (B) Representative images of ARS and ALP staining. Scar bar = 100 μm. (C) Representative RUNX2 immunofluorescence staining image. Scar bar = 100 μm. Gene and protein expression of osteogenic markerslivergent, ALP, OPN, RUNX2 and Colla1, and circ_AFF4, FNDC5 were detected by (D) RT-qPCR and (E) western blot. (F) ELISA detected Irisin expression. *p < 0.05, **p < 0.001 and ***p < 0.001. Each experiment was performed at least three times independently.