Fig. 6
From: CircRNA AFF4 induced by KDM1A promotes osteogenic differentiation through FNDC5/Irisin pathway
KDM1A directly binds to circ_AFF4 and FNDC5 to promote FNDC5/Irisin expression
(A) Schematic illustration showing binding sites between circ_AFF4 promoter and KDM1A predicted by AnimalTFDB, (B) ChIP assay showing the enrichment of BS1-5 sites of circ_AFF4 promoter in complexes precipitated with antibody against KDM1A. (C) ChIP assay showing the impact of KDM1A knockdown on KDM1A/circ_AFF4 promoter interaction. (D) Schematic illustration showing binding sites between FNDC5 promoter and KDM1A predicted by AnimalTFDB, (E) ChIP assay showing the enrichment of BS1-8 sites of FNDC5 promoter in complexes precipitated with antibody against KDM1A. (F) ChIP assay showing the impact of KDM1A knockdown on KDM1A/FNDC5 promoter interaction. Circ_AFF4, FNDC5 and KDM1A were detected by (G) RT-qPCR and (H) western blot. (I) ELISA detected Irisin expression. *p < 0.05, **p < 0.001 and ***p < 0.001. Each experiment was performed at least three times independently