Table 1 The primers in qRT-PCR
From: CircRNA AFF4 induced by KDM1A promotes osteogenic differentiation through FNDC5/Irisin pathway
Gene | Sequence | |
|---|---|---|
ALP | Forward | 5´-GCTGTAAGGACATCGCCTACCA-3´ |
Reverse | 5´-CCTGGCTTTCTCGTCACTCTCA-3´ | |
OPN | Forward | 5´-CGAGGTGATAGTGTGGTTTATGG-3´ |
Reverse | 5´-GCACCATTCAACTCCTCGCTTTC-3´ | |
RUNX2 | Forward | 5´-CGGAATGCCTCTGCTGTTAT-3´ |
Reverse | 5´-TTCCCGAGGTCCATCTACTG-3´ | |
Colla11 | Forward | 5´-CCTGGATGCCATCAAAGTCT-3´ |
Reverse | 5´-AATCCATCGGTCATGCTCTC-3´ | |
circ_AFF4- | Forward | 5´-AAAGGCCAGCATGGATCAGAA-3´ |
convergent | Reverse | 5´-GTGATTTGGAGCGTTGATGTTC-3´ |
circ_AFF4-divergent | Forward | 5´-GCATCGGTTTCTGGTGATGT-3´ |
Reverse | 5´-CGGTTCATGTTGCTTAGTTG-3´ | |
KDM1A | Forward | 5´-TCAGGAGTTGGAAGCGAATCCC-3´ |
Reverse | 5´-GTTGAGAGAGGTGTGGCATTAGC-3´ | |
FNDC5 | Forward | 5´-GTGGTGAGCTGGGATGTTCT-3´ |
Reverse | 5´-GCCTGCACGTGGACTATGTA-3´ | |
GAPDH | Forward | 5´-CGACAGCAGCCGCATCTT-3´ |
Reverse | 5´-CCAATACGACCAAATCCGTTG-3´ |