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Fig. 1 | Molecular Medicine

Fig. 1

From: Bone marrow mesenchymal stem cell-derived exosomes shuttling miR-150-5p alleviates mechanical allodynia in rats by targeting NOTCH2 in microglia

Fig. 1

Identification of BMSCs and BMSC-exosomes. A Morphology of the third-generation BMSCs; B Growth curve of the third-generation BMSCs; C Flow cytometry measured the expression of surface markers (CD90, CD73, CD29, CD105, CD44, CD34, and CD45) in BMSCs; D Alizarin Red staining assessed the osteogenic differentiation of BMSCs, Oil red O evaluated the adipogenic differentiation of BMSCs, and Alcian blue staining revealed the chondrogenic differentiation of BMSCs; E The morphology of the exosomes extracted from BMSCs was observed by transmission electron microscope; F Nanoparticle tracking analysis of BMSC-exosomes; G Western blotting measured the expression of CD81, CD63, CD9, and Calnexin in BMSC-exosomes. BMSC, bone marrow mesenchymal stem cell

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