Fig. 3

Development of AIA in wild-type and SIGIRR-deficient C57BL/6 mice. A Knee joint swelling curves of WT and SIGIRR KO mice (n = 4–5) during AIA. The development of arthritis was monitored daily by measuring knee joint swelling. B H&E staining of joints from WT and SIGIRR KO mice challenged with mBSA; scale bar, 100 μm. C, D Flow cytometry density plots of myeloid cells gated on live single cells and granulocyte or macrophage subsets gated on myeloid cells in synovial tissues with quantification of results as absolute number per inflammatory articulate (i.e., D) or frequency among gated cells (D). E Immunohistochemistry images of joint sections stained with CD4 T cells; scale bar, 20 μm. F CD4-positive cells quantitated by counting brown dots from four to five blind different sections per mouse as shown in E. G Knee joint swelling curves of Rag1^−/− mice transferred with CD4 T cells from WT (WT to Rag1 ^−/−) or SIGIRR KO (KO to Rag1 ^−/−) mice (n = 4) during AIA. H H&E staining of joints in Rag1^−/− mice adoptively transferred with CD4 T cells. Data are representative of two independent experiments, scale bar 100 μm