Fig. 1
From: A novel Mcl-1 inhibitor synergizes with venetoclax to induce apoptosis in cancer cells
MI-238 targets Mcl-1 and disrupts Mcl-1/Bak association. A Chemical information and structure modeling of MI-238 in the BH3-binding pocket of Mcl-1 (PDB ID: 4HW3). B Purification of recombinant GST or GST-Mcl-1 proteins. C Fluorescence polarization (FP) assay was performed to measure the binding of MI-238 to recombinant GST-Mcl-1 protein. Data are represented as mean ± SD, n = 3. D GST pull down assay was conducted to analyze the association between recombinant GST-Mcl-1 (rGST-Mcl-1) and recombinant Bak (rBak) in presence of increasing concentrations of MI-238