Fig. 3
From: A novel Mcl-1 inhibitor synergizes with venetoclax to induce apoptosis in cancer cells
MI-238 effectively induces apoptosis in AML cells. A The cell viability of indicated 8 AML cell lines in presence of increasing concentrations of MI-238 were analyzed by cell counting kit-8 (CCK8) assay and the half-maximal inhibitory concentration (IC50) IC50 were determined. B Western blot analysis of the expression of Mcl-1, Bcl-2 and Bcl-xL in AML cell lines. C Correlation of Mcl-1 protein level and the IC50 of MI-238 were determined. D, E, F Molm13 and MV-4-11 cells were treated with increasing concentrations of MI-238 for 48 h, and the cell apoptosis was analyzed by western blot analysis of PARP1 and caspase 3 cleavage (D) and flow cytometry of annexin V staining (E, F). Data are represented as mean ± SD from three independent replicates, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by two-tailed t-test