Fig. 1

A Photographic image of non-woven fabric polyethylene-polypropylene (PE-PP) disc coated with hydroxyapatite used for isolation and culture of human adipose tissue derived stem cells (hADSCs) without enzymatic digestion. B Representative histogram and dot plot images of the flow cytometric analysis of isolated and cultured hADSCs (2nd passage) for the expression of CD73, CD90, and CD105 and a cocktail of negative markers (CD34, CD11b, CD19, CD45, and HLA-DR) after harvested using Accutase cell detachment solution and treatment with respective anti-human mAbs conjugated with various fluorescent tags. The gated cells in A +—column indicates they are positive for the respective surface marker and the cells in A– column indicates that the negative markers are not expressed in hADSCs. Practically no cells are present in A +—column with the expression of negative markers. C Graphical presentation of the flow cytometry data for the expression of surface markers in cultured hADSCs, human ADSCs from Lonza, and human dermal fibroblast cells. The data are mean ± S.D. of 5 independent experiments