Fig. 4
From: Tetrandrine inhibits RANKL-induced osteoclastogenesis by promoting the degradation of TRAIL
TRAIL overexpression or silencing can promote and inhibit osteoclast differentiation and bone resorption, respectively. A, B The number and area of TRAP-positive BMMs following transfection with TRAIL vector or siTRAIL; scale bar = 200 μm. C–E The bone resorption pits of BMMs following transfection with siTRAIL or TRAIL vector observed with SEM; scale bar = 500 μm. The pit zone resorption area were shown by the ImageJ. F, G The effect of siTRAIL and TRAIL vector on the F-actin ring formation; scale bar = 200 μm. All experiments were carried out in triplicate. The results were presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.005 and ****P < 0.001 as compared with the control group